منابع مشابه
Restoration of phosphate transport by the phosphate-binding protein in spheroplasts of Escherichia coli.
Reconstitution of phosphate transport in Escherichia coli was demonstrated. Conversion of E. coli K10 cells to spheroplasts decreased phosphate transport to about 2%. Addition of purified phosphate-binding protein at physiological levels to these spheroplasts caused a mean 14-fold increase in phosphate transport rate. Crude shock fluid fractions were also stimulatory but not if the shock fluid ...
متن کاملCation Transport in Escherichia coli
The present study is concerned with the measurement of the unidirectional K flux in E, coli. Methods are described by means of which a fairly dense suspension of cells may be maintained in a well defined steady-state with respect to the intracellular K concentration and the pH of the medium. The kinetics of K 4~ exchange under these conditions are consistent with the presence of a single intrac...
متن کاملMagnesium transport in Escherichia coli.
Escherichia coli has a specitic active transport system for magnesium which can be studied with the radioisotope 28Mg. The accumulation of 2*Mg is temperature-dependent and inhibited by cyanide, dinitrophenol, and m-chlorophenyl carbonylcyanide hydrazone. The initial rate of uptake of SMg shows typical saturation kinetics with a & = 18 /LM magnesium and a V,,,,, = 0.56 pmole per min per 1012 ce...
متن کاملCation Transport in Escherichia coli
K influx and net K flux have been measured in suspensions of chloramphenicol-arrested Escherichia coli. The rate of K exchange in the steady state was independent of the K concentration of the medium over a 200-fold range. Under a number of experimental conditions the rate of exchange may be considerably increased or decreased without changing the cellular K content. These results show that und...
متن کاملRole of the phosphotransferase system in Escherichia coli strains deficient in hexose phosphate transport.
In Escherichia coli, glucose 6-phosphate is transported via the Uhp system which is inducible by glucose 6-phosphate. We showed that, in a uhp-deficient strain, glucose 6-phosphate was dephosphorylated in the periplasm and that the resulting glucose was subsequently transported into the cells via the phosphotransferase system. The uptake of glucose generated from glucose 6-phosphate allowed the...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Biomembranes
سال: 1971
ISSN: 0005-2736
DOI: 10.1016/0005-2736(71)90048-4